High Level Expression of Proopiomelanocortin in E. coli Cells using Optimized Codons
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چکیده
منابع مشابه
Optimization and High Level Production of Recombinant Synthetic Streptokinase in E. coli Using Response Surface Methodology
Streptokinase (SK) is an extracellular protein comprising 414 amino acids with considerable clinical importance as a commonly used thrombolytic agent. Due to its wide spread application and clinical importance designing more efficient SK production platforms worth investigatinginvestigation. In this regard, a synthetic SK gene was optimized and cloned in to pET21b plasmid for periplasmic expres...
متن کاملOptimization and High Level Production of Recombinant Synthetic Streptokinase in E. coli Using Response Surface Methodology
Streptokinase (SK) is an extracellular protein comprising 414 amino acids with considerable clinical importance as a commonly used thrombolytic agent. Due to its wide spread application and clinical importance designing more efficient SK production platforms worth investigatinginvestigation. In this regard, a synthetic SK gene was optimized and cloned in to pET21b plasmid for periplasmic expres...
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Interleukin-2 (IL-2), a potent cytokine has been used in anti-cancer therapy for over a decade now. IL-2, originally identified as a growth factor for T lymphocytes is a 15 kDa hydrophobic glycoprotein that induces the activation, clonal proliferation and differentiation of T and B-lymphocytes and enhances the cytotoxicity of monocytes and natural killer (NK) cells. Here, we report a simple met...
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The relationship between structure and function of the nucleosome is an area of exciting and intense research, exemplified by the recent report of the first high-resolution crystallography data (5). The nucleosome, the basic unit of chromatin, consists of DNA wrapped around the core histones H3, H4, H2A and H2B. This structure serves not only to package and organize the two meters of DNA found ...
متن کاملHigh-level expression of tetanus toxin fragment C in Escherichia coli
Fragment C is the C-terminal domain of the heavy chain of tetanus toxin that can promote the immune response against the lethal dose of this toxin. Therefore, this portion can be considered as a candidate vaccine against tetanus infection, which occurs by Clostridium tetani. The present study aimed to compare the expression of tetanus toxin fragment C in Escherichia coli BL21 (DE3) pLysS cells...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2012
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2011.11.276